GFP Self-Amplifying RNA expresses blue fluorescent protein in cells, emitting bright green fluorescence (505/525nm). It’s used for visualizing cellular processes, gene expression analysis, protein localization, and multiplex imaging with other markers. As a self-amplifying RNA, it provides enhanced and prolonged protein expression with lower doses compared to traditional mRNA, making it highly efficient for applications like live cell tracking and gene editing research, where extended observation periods are beneficial. This transient system is ideal for experiments requiring temporary but robust protein expression.
Croyez’s GFP saRNA was generated through in vitro transcription, and these mRNAs are then fortified at their 5' end by modified nucleotide capping, known as Cap1. To mimic the characteristics of fully processed mature mRNAs, we incorporate a poly(A) tail at the 3' end and optimize the mRNAs to enhance stability and overall performance. This ensures that the mRNAs function similarly to naturally occurring mature mRNAs in cells.